A longer account of Dr. Chilton's testimony at the John C. Colt trial.
New York Tribune, January 24, 1842, Page 1, Column 1
Colt's Trial
Fourth Day
Court of Oyer and Terminer
[...]
Dr. Chilton was called. Am a practical
chemist. I called at Colt's room Sept. 24th;
examined it particularly. My attention was first
directed to a spot on the floor which had been
oiled over. On the west wall I observed several
small spots an eighth of an inch in diameter. I
removed and preserved them for examination. I
saw none on the base. There were spots very
small on the folding doors--an immense number
of them, though very minute. I took a small
hatchet, the one now produced, and a piece
of the floor, where the oil had been. I applied
chemical tests, and so far as they went, the spots
were proved to be blood: those from the wall, and
that from the hammer end of the hatchet, and some
which I removed from the eye of the hatchet.
It had the appearance of being inked over, and
on holding it to the light a red appearance may
be observed where the handle joins the hatchet;
I made an examination of the dust settled in the
crease of the floor and it gave indications of blood.
I did not closely examine the parts where the oil
was, yet there was a red appearance under the
oil. I received a piece of newspaper from Justice
Taylor; which had stain on it, (it was here
exhibited to the Court and Jury; it was very much
stained and had holes through it.) The particles
proved to be blood; the paper is dated June 13th,
1841; is a piece of the N. Y. Herald. A piece of
floor is in my hand, (exhibited.) It is about a
foot long and five or six inches wide.
[One of the jurors wished to see the hatchet,
which was handed to him.]
I took also a key and a pen-knife from officer
Smith, which had nothing on them. I have no
doubt that blood was on the articles I have
mentioned.
Cross-examined by Mr [Dudley] Selden.--The largest
spots were taken from the west side of the room,
four or five feet from the floor; I took them off
carefully: and had there been lime in it I still
could have made the analysis. I was requested
by the Mayor to make this analysis. I examined
nothing else by request than what I have
mentioned, as I recollect. There were spots taken
from the wall which were evidently not blood.
The human blood is nearly the same in all men;
but there has been said to be a little difference in
the amount of fibrin in the blood of males and that
of females, but that would not have been disclosed
by my examination. In small quantities there is
no difference presented. It has been said that by
the action of sulphuric acid, different odors might
be perceived in the blood of different animals; but
I have never observed it. The quantity I
examined did not exceed two grains, and I could not
decide whether it was human blood or not.
[...]
----end
Summary of methods used to test for the presence of blood.
The New York Medical and Physical Journal, Volume 7, 1828, Page 309
Method of recognising the stains caused by blood on steel instruments.— [Journal des Progres des Sciences, I. iv. 1827.] Professor Orfila has recently made some experiments on this subject with the view of illustrating certain questions in Medical Jurisprudence,—-in which many doubts are entertained whether stains on steel instruments are caused by blood, by acid juices, or by rust; and the following are the criterion which he proposes. 1. When the instrument is heated to 80 or 90° F. the stain becomes brighter if it is caused by blood, but is not altered if it is caused by rust or lemon juice. 2. A drop of hydrochloric does not alter a blood stain, but dissolves rust or acid stains. 3. The blood-stain steeped in water parts with its colouring matter to that fluid, and the red colour is retained on nitration, while stains caused by rusting or by vegetable acids either do not tinge water at all, or, if they cause a reddish-brown muddiness, it is removed by filtration. 4. The colouring matter derived from the blood stain may likewise be recognised unequivocally by its chemical properties, and in particular by the effect of chlorine: A minute quantity of chlorine turns it green, a larger quantity decolorizes it altogether, and an infusion of galls added to the decolorized solution causes a dark-red precipitate, which is the colouring matter in union probably with tannin. 5. Strong nitric acid destroys the colour of the stain caused by blood; the diluted acid dissolves it, forming a red solution, which precipitates red with infusion of galls.
----end
Account of the sulphuric acid "smell tested" mentioned by Chilton during cross.
The New-York Medical and Physical Journal, Volume 9, January, 1830, Pages 427-429
On the Aromatic Principle of the Blood, and the differences it presents in different Animals and different Sexes.—-In the number of this Journal for January, 1828, some notice was taken of the mode of determining by chemical analysis whether stains on clothes, knives, or other objects suspected to have been produced by blood are really such, or proceed from other causes. The tests which were described on that occasion, and which were ascertained by professor Orfila, it now appears may be applied successfully after a lapse of months or even years. M. Barruel, however, advances a step farther, and maintains that, by means of an aromatic principle, which he conceives exists in the blood, the chemist may distinguish whether blood has proceeded from the human subject or from one of the lower animals, what the animal is, from which it has proceeded,—-and whether, in the case of human blood, it is that of a man or a woman. He says that some years ago, when he was assisting M. Wauquelin in some of his experiments, he remarked, that when sulphuric acid was poured on the crassamentum of ox's blood, a strong odor of a cow house was exhaled. More lately in analyzing the blood of a man who had poisoned himself with opium, he also remarked, that when sulphuric acid was heated in a matrass to ebullition with the blood, an odor of the sweat of the human male was discharged, of such strength, as to compel him to quit the apartment. This circumstance having brought the former fact to his recollection, he proceeded to make further experiments on the subject, and obtained the following results.
The blood of every animal contains a peculiar aromatic principle, which has the same odor with its sweat and pulmonary exhalation. This principle, while it exists in combination with the blood, has no odor; but when the state of combination is broken, it is volatilized, and then the species of animal may be recognized by the odor evolved. The principle has the most powerful odor in the male of each species. It may be developed either from the entire blood, or after the fibrine has been separated, or from the serosity. The best mode of developing the odor is to pour a few drops of blood into a glass, to add between a third part and the half of its volume of concentrated sulphuric acid, and to stir the mixture with a glass rod; upon which the odor will at once be disengaged. By this simple method M. Barruel detected in the blood of man the strong smell of the male human sweat, such as could be confounded with nothing else; in the blood of woman he found the same odor, but much more feeble; in that of the ox a strong odor of a cow stable or cow dung; in that of the horse a strong odor of horse's sweat or horse dung; in that of the ewe a distinct odor of wool impregnated with its oil; in that of the ram an analogous odor mixed with a strong odor of the goat; in that of the dog the odor of its transpiration; in that of the pig a disagreeable smell of a pig stye; and in the blood of the rat the disagreeable smell of that animal. Analogous results were obtained with the blood of fowls, turkeys, ducks, and pigeons; the blood of a frog gave out a distinct smell of marsh rushes; and that of the carp an odor resembling the smell of the slime which covers fresh water fishes. He farther found, that even after blood had dried on cloth, and remained there for fifteen days, the aromatic principle might be disengaged by moistening the stained portion of the cloth and subjecting it in a glass to the action of sulphuric acid.
The experiments of M. Barruel have been repeated by various chemists in France, some of whom were unable to procure the results at which he arrived, while others procured analogous results as to many points, though not in every department of the subject. It appears, for example, that the odor exhaled by the blood of men is sometimes not distinguishable from that of women; for it is always strongest in people who have dark hair, so that the aromatic principle in the blood of a dark haired female, and that of a light haired inale, do not differ materially. M. Soubeiran, who makes this correction on M. Barruel's conclusions, adds, that he could not always satisfy himself that the smell of the aromatic principle of the blood of different animals was specific and characteristic of the animal. M. Raspail denies the accuracy of M. Barruel's statements altogether. M. Villermé was equally unsuccessful with M. Raspail. M. Leuret goes along with M. Barruel in almost all his propositions, and M. Chevallier seems to have obtained results equally concordant. A remarkable fact is stated by M. Leuret. He sent to M. Barruel the blood of a man, of a woman, of a horse, and of an ox, contained in numbered phials; and M. Barruel distinguished among the blood of the two last, and pronounced that both the two first were human blood; but he inferred, from his experiments, that the male blood was that of a woman, and the female blood that of a man. This error was afterwards easily explained: The woman from whom the blood was taken was dark and of a strong frame, while the man was of a lymphatic temperament, with delicate skin, and no hair on the face. These criticisms on M. Barruel's discovery are contained in the Annales d'Hygiene, &c., for October last, and in the Revue Medicale for September. It is added, in explanation of the success of M. Barruel, and the failure of others, that this gentleman has a peculiarly delicate sense of smell. The whole subject is well worthy of farther investigation, as promising to supply the medical jurist with a very useful instrument af research in judicial cases.—-Annales d'Hygiene Publique et de Médecine Légale, Avril, 1829.
----end
Link to an 1836 forensic medicine text with a section on blood spots. Only one volume was published.
Elements of Medical Jurisprudence, Volume I (London: Deacon, 1836), link
By Alfred Swaine Taylor
Pages 376-389
Identification of Spots of Blood
A later edition.
Elements of Medical Jurisprudence (London: John Churchill, 1844), link
By Alfred Swaine TAYLOR
Pages 332-338
Chemical Examination of Blood Stains
An abridged, American translation of Orfila's text on toxicology.
A General System of Toxicology: or, A Treatise on Poisons (Philadelphia: M. Carey & Son, 1817), link
by Matthieu Joseph Bonaventure Orfila, translated by John Augustine Waller
Orfila was known for his involvement in the case of Madame Lafarge, accused of poisoning her husband. A link to an account of her trial.
The Lancet, Volume 1, December 26, 1840, Pages 479-482
Trial of Madame Lafarge
New York Tribune, January 24, 1842, Page 1, Column 1
Colt's Trial
Fourth Day
Court of Oyer and Terminer
[...]
Dr. Chilton was called. Am a practical
chemist. I called at Colt's room Sept. 24th;
examined it particularly. My attention was first
directed to a spot on the floor which had been
oiled over. On the west wall I observed several
small spots an eighth of an inch in diameter. I
removed and preserved them for examination. I
saw none on the base. There were spots very
small on the folding doors--an immense number
of them, though very minute. I took a small
hatchet, the one now produced, and a piece
of the floor, where the oil had been. I applied
chemical tests, and so far as they went, the spots
were proved to be blood: those from the wall, and
that from the hammer end of the hatchet, and some
which I removed from the eye of the hatchet.
It had the appearance of being inked over, and
on holding it to the light a red appearance may
be observed where the handle joins the hatchet;
I made an examination of the dust settled in the
crease of the floor and it gave indications of blood.
I did not closely examine the parts where the oil
was, yet there was a red appearance under the
oil. I received a piece of newspaper from Justice
Taylor; which had stain on it, (it was here
exhibited to the Court and Jury; it was very much
stained and had holes through it.) The particles
proved to be blood; the paper is dated June 13th,
1841; is a piece of the N. Y. Herald. A piece of
floor is in my hand, (exhibited.) It is about a
foot long and five or six inches wide.
[One of the jurors wished to see the hatchet,
which was handed to him.]
I took also a key and a pen-knife from officer
Smith, which had nothing on them. I have no
doubt that blood was on the articles I have
mentioned.
Cross-examined by Mr [Dudley] Selden.--The largest
spots were taken from the west side of the room,
four or five feet from the floor; I took them off
carefully: and had there been lime in it I still
could have made the analysis. I was requested
by the Mayor to make this analysis. I examined
nothing else by request than what I have
mentioned, as I recollect. There were spots taken
from the wall which were evidently not blood.
The human blood is nearly the same in all men;
but there has been said to be a little difference in
the amount of fibrin in the blood of males and that
of females, but that would not have been disclosed
by my examination. In small quantities there is
no difference presented. It has been said that by
the action of sulphuric acid, different odors might
be perceived in the blood of different animals; but
I have never observed it. The quantity I
examined did not exceed two grains, and I could not
decide whether it was human blood or not.
[...]
----end
Summary of methods used to test for the presence of blood.
The New York Medical and Physical Journal, Volume 7, 1828, Page 309
Method of recognising the stains caused by blood on steel instruments.— [Journal des Progres des Sciences, I. iv. 1827.] Professor Orfila has recently made some experiments on this subject with the view of illustrating certain questions in Medical Jurisprudence,—-in which many doubts are entertained whether stains on steel instruments are caused by blood, by acid juices, or by rust; and the following are the criterion which he proposes. 1. When the instrument is heated to 80 or 90° F. the stain becomes brighter if it is caused by blood, but is not altered if it is caused by rust or lemon juice. 2. A drop of hydrochloric does not alter a blood stain, but dissolves rust or acid stains. 3. The blood-stain steeped in water parts with its colouring matter to that fluid, and the red colour is retained on nitration, while stains caused by rusting or by vegetable acids either do not tinge water at all, or, if they cause a reddish-brown muddiness, it is removed by filtration. 4. The colouring matter derived from the blood stain may likewise be recognised unequivocally by its chemical properties, and in particular by the effect of chlorine: A minute quantity of chlorine turns it green, a larger quantity decolorizes it altogether, and an infusion of galls added to the decolorized solution causes a dark-red precipitate, which is the colouring matter in union probably with tannin. 5. Strong nitric acid destroys the colour of the stain caused by blood; the diluted acid dissolves it, forming a red solution, which precipitates red with infusion of galls.
----end
Account of the sulphuric acid "smell tested" mentioned by Chilton during cross.
The New-York Medical and Physical Journal, Volume 9, January, 1830, Pages 427-429
On the Aromatic Principle of the Blood, and the differences it presents in different Animals and different Sexes.—-In the number of this Journal for January, 1828, some notice was taken of the mode of determining by chemical analysis whether stains on clothes, knives, or other objects suspected to have been produced by blood are really such, or proceed from other causes. The tests which were described on that occasion, and which were ascertained by professor Orfila, it now appears may be applied successfully after a lapse of months or even years. M. Barruel, however, advances a step farther, and maintains that, by means of an aromatic principle, which he conceives exists in the blood, the chemist may distinguish whether blood has proceeded from the human subject or from one of the lower animals, what the animal is, from which it has proceeded,—-and whether, in the case of human blood, it is that of a man or a woman. He says that some years ago, when he was assisting M. Wauquelin in some of his experiments, he remarked, that when sulphuric acid was poured on the crassamentum of ox's blood, a strong odor of a cow house was exhaled. More lately in analyzing the blood of a man who had poisoned himself with opium, he also remarked, that when sulphuric acid was heated in a matrass to ebullition with the blood, an odor of the sweat of the human male was discharged, of such strength, as to compel him to quit the apartment. This circumstance having brought the former fact to his recollection, he proceeded to make further experiments on the subject, and obtained the following results.
The blood of every animal contains a peculiar aromatic principle, which has the same odor with its sweat and pulmonary exhalation. This principle, while it exists in combination with the blood, has no odor; but when the state of combination is broken, it is volatilized, and then the species of animal may be recognized by the odor evolved. The principle has the most powerful odor in the male of each species. It may be developed either from the entire blood, or after the fibrine has been separated, or from the serosity. The best mode of developing the odor is to pour a few drops of blood into a glass, to add between a third part and the half of its volume of concentrated sulphuric acid, and to stir the mixture with a glass rod; upon which the odor will at once be disengaged. By this simple method M. Barruel detected in the blood of man the strong smell of the male human sweat, such as could be confounded with nothing else; in the blood of woman he found the same odor, but much more feeble; in that of the ox a strong odor of a cow stable or cow dung; in that of the horse a strong odor of horse's sweat or horse dung; in that of the ewe a distinct odor of wool impregnated with its oil; in that of the ram an analogous odor mixed with a strong odor of the goat; in that of the dog the odor of its transpiration; in that of the pig a disagreeable smell of a pig stye; and in the blood of the rat the disagreeable smell of that animal. Analogous results were obtained with the blood of fowls, turkeys, ducks, and pigeons; the blood of a frog gave out a distinct smell of marsh rushes; and that of the carp an odor resembling the smell of the slime which covers fresh water fishes. He farther found, that even after blood had dried on cloth, and remained there for fifteen days, the aromatic principle might be disengaged by moistening the stained portion of the cloth and subjecting it in a glass to the action of sulphuric acid.
The experiments of M. Barruel have been repeated by various chemists in France, some of whom were unable to procure the results at which he arrived, while others procured analogous results as to many points, though not in every department of the subject. It appears, for example, that the odor exhaled by the blood of men is sometimes not distinguishable from that of women; for it is always strongest in people who have dark hair, so that the aromatic principle in the blood of a dark haired female, and that of a light haired inale, do not differ materially. M. Soubeiran, who makes this correction on M. Barruel's conclusions, adds, that he could not always satisfy himself that the smell of the aromatic principle of the blood of different animals was specific and characteristic of the animal. M. Raspail denies the accuracy of M. Barruel's statements altogether. M. Villermé was equally unsuccessful with M. Raspail. M. Leuret goes along with M. Barruel in almost all his propositions, and M. Chevallier seems to have obtained results equally concordant. A remarkable fact is stated by M. Leuret. He sent to M. Barruel the blood of a man, of a woman, of a horse, and of an ox, contained in numbered phials; and M. Barruel distinguished among the blood of the two last, and pronounced that both the two first were human blood; but he inferred, from his experiments, that the male blood was that of a woman, and the female blood that of a man. This error was afterwards easily explained: The woman from whom the blood was taken was dark and of a strong frame, while the man was of a lymphatic temperament, with delicate skin, and no hair on the face. These criticisms on M. Barruel's discovery are contained in the Annales d'Hygiene, &c., for October last, and in the Revue Medicale for September. It is added, in explanation of the success of M. Barruel, and the failure of others, that this gentleman has a peculiarly delicate sense of smell. The whole subject is well worthy of farther investigation, as promising to supply the medical jurist with a very useful instrument af research in judicial cases.—-Annales d'Hygiene Publique et de Médecine Légale, Avril, 1829.
----end
Link to an 1836 forensic medicine text with a section on blood spots. Only one volume was published.
Elements of Medical Jurisprudence, Volume I (London: Deacon, 1836), link
By Alfred Swaine Taylor
Pages 376-389
Identification of Spots of Blood
A later edition.
Elements of Medical Jurisprudence (London: John Churchill, 1844), link
By Alfred Swaine TAYLOR
Pages 332-338
Chemical Examination of Blood Stains
An abridged, American translation of Orfila's text on toxicology.
A General System of Toxicology: or, A Treatise on Poisons (Philadelphia: M. Carey & Son, 1817), link
by Matthieu Joseph Bonaventure Orfila, translated by John Augustine Waller
Orfila was known for his involvement in the case of Madame Lafarge, accused of poisoning her husband. A link to an account of her trial.
The Lancet, Volume 1, December 26, 1840, Pages 479-482
Trial of Madame Lafarge
Comment